Mudit Tyagi



Editor Name: Mudit Tyagi

 

Country: USA

 

University: Thomas Jefferson University

 

Educational Qualification: PhD, Molecular Virology

 

Designation: Associate Professor

 

Department: Department of molecular Medicine

 

Email:  mxt108[@]gmail[.]com ; mudit.tyagi[@]Jefferson[.]edu

 

Research Interest: HIV, HIV latency, HIV Transcription, HIV Replication, Epigenetics, Drugs of abuse, Cocaine, Biochemistry.

 

BIOGRAPHY: 


He is a enthusiastic about both laboratory-oriented and theoretical courses. I taught Microbiology and Immunology course at Case Western Reserve University to M.D. / PhD students. I also worked as Adjunct Faculty at Cuyahoga Community College, Cleveland, there I taught Microbiology course to undergraduates. I have trained several undergraduate, postgraduate and post-doctoral fellows as assistant professor at the George Washington University (GWU). That experience helped me to realize my love for teaching. I also enjoy one-on-one teaching and advising. My teaching interests are similarly broad. I would be interested in teaching Biochemistry, Microbiology, Molecular Biology, immunology, cell biology and also course regarding lab techniques. All of them I can teach comfortably at both undergraduate and graduate levels.

EDUCATION:

 

PhD, Molecular Virology:

Department of molecular Medicine, International Center for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy

Dissertation: “Transcriptional and Non Transcriptional Properties of the Tat Protein of HIV-1” defended in December. Mentor: Dr. Mauro Giacca

MSc, Biochemistry:

Department of Biochemistry, Indian Veterinary Research Institute (IVRI), Izatnagar, Bareily, India

Dissertation: “Characterization of Immunogenic Protein Gene of Foot and Mouth Disease Virus Type ASIA-1, 63/72.” defended in June.

Mentor: Dr. VVS Suryanarayana

Post-graduate Diploma, Biochemical Technology: Delhi University, Delhi, India

BSc (Honors), Biochemistry:  Delhi University, Delhi, India

 

POSITIONS AND HONORS:

2018 - Present Associate Professor, Center for Translational Medicine, Thomas Jefferson University, Philadelphia, USA

2012 - 2018 Assistant Professor, Department of Medicine; Department of Microbiology, Immunology and Tropical medicine, George Washington University, Washington DC, USA

2011 - 2012 Assistant Professor Research, National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, USA

2009 - 2010 Instructor, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA

2008 - Present Adjunct faculty at Cuyahoga Community College, Cleveland, Ohio

2002 - 2009 Research Associate, School of Medicine, Case Western Reserve University, Cleveland, Ohio, USA

2002 - 2002 Senior Research Fellow, Medical Research Council (MRC) Laboratory of Molecular Biology, Cambridge, UK

1999 - 2002 Post-doctoral Fellow, International Center for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy

1995 - 1999 Pre-doctoral Fellowship from International Center for Genetic Engineering and Biotechnology (ICGEB), Trieste, Italy

1992 - 1994 Junior research fellowship from Indian veterinary Research Institute (IVRI), Bareilly, India

PUBLICATIONS:

 

1. Meltzer B, Dabbagh D, Guo J, Tyagi M and Yuntao Wu; Tat Controls Transcriptional Persistence of Unintegrated HIV Genome in Primary Human Macrophages. Virology. 2018 Mar 14; 518:241-252. doi: 10.1016/j.virol.2018.03.006. [Epub ahead of print], PMID:29549786.

 

2. Sonia Zicari1,2#, Geetaram Sahu1#, Larisa Dubrovsky3, Lin Sun1,Han Yue1, Tejaswi Jada1, Alex Ochem4, Michael Bukrinsky3, Gary Simon1 and Mudit Tyagi1, 3*; DNA-PK facilitates HIV transcription by regulating the activity of RNA polymerase II and the recruitment of transcription machinery at HIV LTR. 2017, under Post-revision stage in Oncotarget BioRvix 174573 [Preprint]; August 10, 2017. Available from: https://doi.org/10.1101/174573

(Corresponding Author).

 

3. Sonia Zicari, Lin Sun, Liam Spurr, Gary Simon and Mudit Tyagi; CBF-1 promotes the establishment and maintenance of HIV latency by recruiting Polycomb repressive complexes, PRC1 and PRC2, at HIV LTR; 2017, under post-revision stage in Virology, BioRvix 174607 [Preprint]; August 10, 2017. Available from: https://doi.org/10.1101/174607, (Corresponding Author).

 

4. Tyagi M1, Bukrinsky M, Simon GL; Mechanisms of HIV Transcriptional Regulation by Drugs of Abuse. Current HIV Research, 2016;14(5):442-454. (Corresponding Author)

 

5. Sahu G, Farley K, El-Hage N, Aiamkitsumrit B, Fassnacht R, Kashanchi F, Ochem A, Simon GL, Karn J, Hauser KF, Tyagi M; Cocaine promotes both initiation and elongation phase of HIV-1 transcription by activating NF-κB and MSK1 and inducing selective epigenetic modifications at HIV-1 LTR. Virology, 2015 September;483;185–202, [Epub ahead of print] (Corresponding Author).

 

6. Mudit Tyagi, Jaime Weber, Michael Bukrinsky and Gary L. Simon; The effects of cocaine on HIV transcription, J Neurovirol; 2016 Jun;22(3):261-74. doi: 10.1007/s13365-015-0398-z. (Corresponding Author).

 

7. Mudit Tyagi, Sergey Iordanskiy, Kahli Smith, Denitra Breuer, Namita Kumari, Andrey V. Ilatovskiy, Tatyana Ammosova, Yasemin SaygideÄŸer Kont, Andrey Ivanov, Aykut Ãœren, Dmytro Kovalskyy, Michael Petukhov, and Sergei Nekhai; Reactivation of Latent HIV-1 Provirus via Targeting Protein Phosphatase-1. Retrovirology. 2015 Jul 16;12(1):63. doi: 10.1186/s12977-015-0190-4.

 

8. Farley, K., El-Hage, N., Fassnacht, R., Hauser, K. Karn, J., Mudit Tyagi, M; Cocaine induces HIV gene expression by activating NF-kB and selective epigenetic modifications, J Neurovirol; 2013 Oct;19:28; 1355. (Corresponding Author)

 

9. Mudit Tyagi and Michael Bukrinsky; HIV latency: The major hurdle in HIV eradication. Molecular Medicine, 2012 Sep 25;18(1):1096-108, (Corresponding Author), Depicted on the cover page of Journal.

 

10. Mudit Tyagi and Fatah Kashanchi; New intrinsic host restriction factors against HIV-1: PAF1 complex, HERC5 and others. Retrovirology, 2012, Mar 9; 9:19. One of the most cited papers of the month.

 

11. Tyagi S, Ochem A, Tyagi M; DNA-dependent protein kinase interacts functionally with the RNA polymerase II complex recruited at the human immunodeficiency virus (HIV) long terminal repeat and plays an important role in HIV gene expression. J Gen Virol. 2011 Jul;92(Pt 7):1710-20. Epub 2011 Mar 30. (Corresponding Author)

 

12. Rachel Van Duyne, Irene Guendel, Mudit Tyagi, Aarthi Narayanan, Zachary Klase, Kylene Kehn-Hall, John Semmes, Fatah Kashanchi; Localization and sub-cellular shuttling of HTLV-1 Tax with the RNAi machinery component Drosha. Retrovirology. 2011; 8(Suppl 1): A159. Published online 2011 Jun 6. doi: 10.1186/1742-4690-8-S1-A159, PMCID: PMC3112630

 

13. Mudit Tyagi and Fabio Romerio; HIV-1 persistence in the CD4+ T cell compartment: past, present and future. Current HIV Research, 2011, Dec 1; 9(8):579-87.

 

14. Duyne RV, Guendel I, Narayanan A, Gregg E, Shafagati N, Tyagi M, Easley R, Klase Z, Nekhai S, Kehn- Hall K; Varying Modulation of HIV-1 LTR Activity by BAF Complexes. J Mol Biol. 2011 Aug 19; 411(3):581-96.

 

15. Matthew S Lalonde, Michael A Lobrtiz, Annette Ratcliff, Zafiria Athanassiou, Mudit Tyagi, Julian Wong, John A Robinson, Jonathan Karn, Gabriele Varani, Eric J. Arts; Inhibition of both HIV-1 reverse transcription and gene expression by a cyclic peptide that binds the Tat-transactivating response element (TAR) RNA. PLoS Pathogen 2011, 7(5), e1002038.

 

16. Rachel Van Duyne, Irene Guendel, Nazly Shafagati, Kylene Kehn-Hall, Rebecca Easley, Zachary Klase, Sergei Nekhai, Mudit Tyagi, and Fatah Kashanchi; Varying modulation of HTLV-1 LTR activity by BAF complexes. Retrovirology, 2011. Published online 2011 June 6.

 

17. Mudit Tyagi, Richard Pearson and Jonathan Karn; Establishment of HIV latency in primary CD4+ cells is due to epigenetic transcriptional silencing and P-TEFb restriction. J Virol. 2010 Jul; 84(13):6425-37.

 

18. Filomena Nappi, Chiara Chiozzini, Valentina Bordignon, Alessandra Borsetti, Stefania Bellino, Marco Cippitelli, Giovanni Barillari, Antonella Caputo, Mudit Tyagi, Mauro Giacca and Barbara Ensoli; Immobilized HIV-1 Tat protein promotes gene transfer via a transactivation-independent mechanism, which requires binding of Tat to

viral particles. J Gene Med.. 2009 Nov; 11(11):955-65

 

19. Masanori Nojima, Yehong Huang, Mudit Tyagi, Hung-Ying Kao and Koh Fujinaga.; The positive transcription elongation factor B is an essential co-factor for the activation of transcription by myocyte enhancer factor 2. J Mol Biol. 2008 Oct 3;382(2):275-87.

 

20. Richard Pearson, Young-Kyeung Kim, Joseph Hokello, Kara Lassen, Julia Friedman, Mudit Tyagi and Jonathan Karn; Epigenetic silencing of HIV transcription through formation of restrictive chromatin structures at the viral LTR drives the progressive entry of HIV into latency. J Virol. 2008 Dec;82(24):12291-303.

 

21. Mudit Tyagi and Jonathan Karn; CBF-1 promotes transcriptional silencing during the establishment of HIV-1 latency. EMBO J. 2007 Dec 12;26(24):4985-95. Epub 2007 Nov 15.

 

22. Kim YK, Cyril F. Bourgeois, Richard Pearson, Mudit Tyagi, Michelle J. West, Julian Wong, Shwu-Yuan Wu, Cheng-Ming Chiang, and Jonathan Karn: Recruitment of TFIIH to the HIV LTR is a rate-limiting step in the emergence of HIV from latency. EMBO J. 2006 Aug 9;25(15):3596-604. Epub 2006 Jul 27.

 

23. Pantano S, Tyagi M, Giacca M, Carloni P. Molecular dynamics simulations on HIV-1 Tat. Eur Biophys J.

2004 Jul;33(4):344-51.

 

24. Pantano S, Tyagi M, Giacca M, Carloni P. Amino acid modification in the HIV-1 Tat basic domain: insights from molecular dynamics and in vivo functional studies. J Mol Biol. 2002 May 17;318(5):1331-9.

 

25. Silhol M, Tyagi M, Giacca M, Lebleu B, Vives Different mechanisms for cellular internalization of the HIV- 1 Tat-derived cell penetrating peptide and recombinant proteins fused to Tat. Eur J Biochem. 2002 Jan;269(2):494-501. (Cited by more than 190 articles)

 

26. Tyagi M, Rusnati M, Presta M, Giacca M.Internalization of HIV-1 Tat Requires Cell Surface Heparan Sulfate Proteoglycans. J Biol Chem. 2001 Feb 2;276(5):3254-3261. (Cited by more than 1000 articles)

 

27. Marzio G*, Tyagi M*, Gutierrez MI, Giacca M. HIV-1 Tat transactivator recruits p300 and CREB-binding protein histone acetyltransferases to the viral promoter. Proc Natl Acad Sci USA 1998 Nov 10;95 (23):13519-24. *First co-Authors contributed equally (Cited by more than 450 articles)

 

28. Tulasiram P, Tyagi M, Srinivas K, Prabhudas K, Natarajan C, Suryanarayana VVS. Antigenic variation in Foot and Mouth Disease Virus type Asia1 isolates circulated during 1993-95. Virus Genes 1997,15 (3): 247-53.

 

29. Cinelli RA, Ferrari A, Pellegrini V, Tyagi M, Giacca M, Beltram F. The enhanced green fluorescent protein as a tool for the analysis of protein dynamics and localization: local fluorescence study at the single- molecule level. Photochem Photobiol. 2000 Jun;71(6):771-6. This article was discussed in several local and national newspapers of Italy.

 

30. Rusnati M, Taraboletti G, Urbinati C, Tulipano G, Giuliani R, Molinari-Tosatti MP, Sennino B, Giacca M, Tyagi M, Albini A, Noonan D, Giavazzi R, Presta M. Thrombospondin-1/HIV-1 tat protein interaction: modulation of the biological activity of extracellular Tat. FASEB J. 2000 Oct;14(13):1917-30.

 

31. Riccardo A. G. Cinelli, Aldo Ferrari, Vittorio Pellegrini, Anna Signorelli, Mudit Tyagi, Mauro Giacca, and Fabio Beltram Engineering Single-Molecule Fluorescence Dynamics for Advanced Biomolecular Applications. Aust. J. Chem. 2001. 54, 107-111.

 

32. Cinelli RA, Tozzini V, Pellegrini V, Beltram F, Cerullo G, Zavelani-Rossi M, De Silvestri S, Tyagi M, Giacca M. Coherent dynamics of photoexcited green fluorescent proteins. Phys Rev Lett. 2001 Apr 9;86(15):3439-42.

 

33. Alessandro Marcello, Riccardo A. G. Cinelli, Aldo Ferrari, Anna Signorelli, Mudit Tyagi, Vittorio Pellegrini, Fabio Beltram and Mauro Giacca Visualization of direct interaction between HIV-1 Tat and human cyclin T1 and its sub-cellular localization. J Biol Chem. 2001 Oct 19;276(42):39220-5.

 

34. Riccardo A. G. Cinelli, Vittorio Pellegrini, Aldo Ferrari, Paolo Faraci, Riccardo Nifosi, Mudit Tyagi, Mauro Giacca and Fabio Beltram Green Fluorescent protein as optically-controllable elements in bioelectronics. Appl.Phys.Lett. 2001 Nov 12; 79: 3353-3355. This article was discussed in several local and national newspapers of Italy. (Cited by more than 300 articles).

 

My Bibliography at NCBI:

https://www.ncbi.nlm.nih.gov/sites/myncbi/mudit.tyagi.1/bibliography/44661777/public/?sort=date&direction=ascending

 

INVITED SPEAKER:

1) Department of Cell and Tissue Biology, University of California, San Francisco, California, 2001.

2) Department of Microbiology, University of Texas Southwestern Medical Center at Dallas, Texas, 2008.

3) Nebraska Center for Virology, University of Nebraska, Lincoln, Nebraska, USA, 2009.

4) International Center for Genetic Engineering and Biotechnology, Trieste, Italy, June, 2010.

5) National Center for Biodefense and Infectious Diseases, George Mason University, Manassas, Virginia, August 2010.

6) Department of Genetic Medicine, University of North Carolina, Chapel Hill, North Carolina, October 2010.

7) Department of Microbiology, Immunology & Tropical Medicine, George Washington University, Washington DC, July 2011.

8) Virginia Universities AIDS Research Consortium conference, Virginia Beach, Virginia, October 7 2011.

9) Dep. of Biochemistry and Molecular Biology, New Jersey Medical School, UMDNJ, Newark, Oct 20, 2011.

10) Virginia Universities AIDS Research Consortium conference, University of Virginia, Virginia, May, 2011.

11) Department of Biomedical Sciences, University of Texas, El Paso, Texas, Fab, 2012.

12) Department of Microbiology and Biology, University of Alaska at Fairbanks, Fairbanks, Alaska, July, 2012.

13) Department of veterinary medicine, Michigan State University, East Lansing, Michigan, Aug, 2012.

14) Virginia Universities AIDS Research Consortium conference, GMU, Fairfax, Virginia, Fab, 2013.

15) Virginia Universities AIDS Research Consortium conference, Virginia Beach, Virginia, March 26, 2015.

16) AmfAR Think Tank, Memphis, Tennessee, March 11-13, 2016

17) ISNV-Chicago, Illinois, April 2018

Journals

American Scientific Reports in Infection Prevention and Control

American Scientific Reports in Infection Prevention and Control

American Scientific Reports in Infection Prevention and Control is an Scholarly Open Access scient

American Scientific Reports in Communicable Diseases

American Scientific Reports in Communicable Diseases

American Scientific Reports in Communicable Diseases is an international peer reviewed journal pub

American Scientific Reports in Clinical Nutrition

American Scientific Reports in Clinical Nutrition

American Scientific Reports in Clinical Nutrition is an international journal providing essential

American Scientific Reports in Mental Health

American Scientific Reports in Mental Health

American Scientific Reports in Mental Health is an International open access journal that publishes

American Scientific Reports in Surgery and Anesthesia

American Scientific Reports in Surgery and Anesthesia

American Scientific Reports in Surgery and Anesthesia is an International open access, peer-revie

American Scientific Reports in Pediatrics

American Scientific Reports in Pediatrics

American Scientific Reports in Pediatrics is an international Open Access, peer-reviewed journal t

American Scientific Reports in Gynecology and Obstetrics

American Scientific Reports in Gynecology and Obstetrics

American Scientific Reports in Gynecology and Obstetrics is an international open access peer-revi

American Scientific Reports in Otolaryngology and Rhinology

American Scientific Reports in Otolaryngology and Rhinology

American Scientific Reports in Otolaryngology and Rhinology is an international, open access, pee

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