The
use of alkylamine ion pair and perfluorinated alcohols in the analysis of
oligonucleotides by LC-MS techniques has been the mainstay in characterisation
and quantitation methods for synthetic oligonucleotides for decades. Often,
analytical chemists default to relatively high concentrations of both, but how
much ion pair is needed for optimal chromatographic performance and how much
does perfluorinated alcohol concentration contribute to electrospray ionisation
efficiency?
In
this on-demand, we provide an overview of nucleic acid therapeutics and discuss
the chromatographic challenges associated with oligonucleotides. We also
explore modulation of the mobile phase additives alkylamine ion pair and
perfluorinated alcohols. Finally, we will show how optimisation of mobile phase
can improve not only chromatographic performance and MS sensitivity, but may
also directly impact MS data acquisition, thus facilitating data analysis.
Learning outcomes of this webinar:
KEYNOTE SPEAKER
Brian Rivera, Senior
Product Manager, Phenomenex
Brian Rivera is currently
the Product Manager for Bioseparations Products at Phenomenex, Incorporated,
Torrance, California, US. Prior to this role, Brian has held a variety of
positions in sales and marketing related to biotechnology products. He worked
as a Technical Sales Specialist for Phenomenex and Eppendorf, an Account
Manager for ProZyme and a Biotechnology Product Specialist for Phenomenex.
Prior to his business-facing roles, Brian worked in analytical methods
development and in manufacturing support roles for a few biotechnology
companies in the San Francisco Bay Area; including Avid Bioservices, ProZyme
and Chiron Corporation. He has a bachelor’s degree from the University of California,
Davis.
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